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-   -   Very Easy Sterilisation Method - My Seedlings (https://www.orchidboard.com/community/propagation/43343-easy-sterilisation-method-seedlings.html)

camille1585 02-02-2011 02:33 PM

Thanks! I just need to find the NAA, otherwise I have everything else. I have Cattleya, Brassavola and Dendrobium seed, so I guess I'll try to make a flask of Den seeds. But I'm in the netherlands and my seeds are in france, so it will have to wait a few months.

Mr. Tech 02-03-2011 04:32 AM

I tried this method today. Hope the best.

roby 02-03-2011 05:09 AM

well, update me about the results, positive or negative. :D

How many hours did you leave the pad



thanks

Jeff9 02-03-2011 06:33 AM

Hi Roby,

Thanks for the information, i wish i had some seed to sow id like to attempt this ;)

A question, why do you not also sterilize the seeds? Is it more hassle or will this much sterilization kill the seeds?

Thanks,
Jeff

roby 02-03-2011 12:27 PM

Hi Jeff
This method is just to disinfect the seeds (see pdf)
Then I adjusted the system for seeding without boxes sterile.
For me, the classic method of disinfection of seeds and sowing is too complicated :D

Phalguy 02-03-2011 02:26 PM

Hi Roby...

I'm about to sow some Phals very soon and I will try your method! I'll keep you posted!
Thanks for sharing

roby 02-03-2011 03:15 PM

Excellent! :D
The most delicate part is when you take away the pad, just lift the top sheet of plastic

Jeff9 02-03-2011 06:29 PM

Once again thanks alot Roby, this technique could potentially completely change the way we cultivate orchids in vitro - if you are the first person to use it for this purpose.

I have a question, did you harvest and pollinate the pods yourself? I have attempted to pollinate orchids alot of times but it never succeeded, any tips?

Phalguy 02-03-2011 07:13 PM

Hello Jeff9

I do pollinate and harvest seeds from my phals...
After a few try, I have better chance when:
1. I cross an old flower on a young flower ( same thing as humain no? ;-) )
2. pollinate in the morning
3. Don't remove the pollen on the mother flower
4. If selfing, flowers should be on 2 different spike
5. Orchid can be very stubborn!

Since I do that, I have almost no fail...

Phalguy 02-03-2011 07:16 PM

Quote:

Originally Posted by roby (Post 377212)
Excellent! :D
The most delicate part is when you take away the pad, just lift the top sheet of plastic

Helo again...

First, what do you mean by that? Second, how big is the hole in the plastic wrap?

thanks! ;-)


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