Just tried again with new seed. It's so simple! Lets see if I did work clean.

Eleven days now and no contamination. That is very good. But still to early to discearn with naked eye if anything moves.

Good luck.

sterilization of homemade media
 

hi i am new here. please anyone help me how to sterilize germinating media with a microwave oven. As far as i got first need to mix all ingredients of the media,then put needed amount of media in jar, keep the lid of jar little open, put the jar in microwave, set the temp in 150C and keep the oven run for 30 minutes. Am i right?

It sounds like you're mixing up oven sterilisation with microwave sterilisation. If you want to sterilise in an oven, then, then that would be about 150C for 30 minutes, and if you're using plastic containers you'll need to make sure they're oven safe.

thank you NatalieS. Would you please tell me that the process i described is proper order or not? what is the difference between ordinery oven and microwave oven sterilization process?

Well, basically the oven takes longer. In both cases you'd make up your medium over the stove first. Many people have written very good information about the different methods of sterilisation, and I doubt I could improve on the info they've provided. I recommend taking a look at this site as a first step:

Lotte & Thomas Orchids

Lots of info about different sterilisation methods, media preparation, etc.

Also, try searching on YouTube for 'microwave sterilisation of orchid medium'. There are some very good videos there.

Lot of thanks NatalieS

please help me with 100% home made media for cattleya / brassavola seed germination. 10 days ago i have sown some den. pulchellum seed in ready made media by Roby 's described method and i am happy still no sign of contamination.only three flask. please pray for success.

here is my first atempt flask. seeds are turning green now.