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Are you replating in a seald jar or flask?

Quote:

Originally Posted by plumania (Post 645048)

Sunlight through magnifying glass might actually burn and poke a hole in the plastic flask. We used to poke holes in plastic and burn small piece of paper during science experiments at home with help of magnifying glass.

If you focus the beam on the plastic wall, yes, that will burn a hole. If you focus the beam on mold that is one or two inches inside the wall and on top of agar, I don't think that there will be damage to the flask.

Anyway, if a flask is infected, it is worth trying this instead of tossing it in the trash.

You can try cotton wool plug in the lid or tape some cotton wool over the hole. But you need to leave a small part untapped over the hole, so the jar can breathe.

Quote:

Originally Posted by OCRC_Dir_China (Post 647365)

You can try cotton wool plug in the lid or tape some cotton wool over the hole. But you need to leave a small part untapped over the hole, so the jar can breathe.

Not necessarily. It has been shown that it is not necessary

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Wow~ this is so cool!!!

How are your seedlings now???

I tried this method. My conclusions : very easy, a lot easier for me than the usual system. 80% success. It worked with laelia, phalaenopsis and ascocenda hybrids. I am very happy with it. :)
Maybe to adapt for disa seeds ; no infection but no germination, maybe the disa seeds are not strong enough for 4h sterilisation...
Thanks a lot to Roby !

I used this method & posted on this thread some time ago. Those tiny seedlings have now been moved to compots, though I never was able to successfully replate with this method. I went from mother flask to deflasking straight away.

As I replate in a glove box from a sterile flask to another sterile flask, I just transfer the small plants without any more sterilisation. It works usually. Only one infection in a flask after 1 month replate, so I deflasked and put the plants (Laelia Purpurata Mandayana) in sphagnum. They are slowly growing now. T find the sterilisation of the disa seeds difficult, so I try to sowe green as much as possible.

Quote:

Originally Posted by NatalieS (Post 542155)

Unfortunately my bellatulum seeds didn't go far beyond germination. I think I was not maintaining the correct conditions. I am however pleased to report successful germination of some bulbo orectopetalum seeds which I have subsequently replated. All using Roby's vapour sterlisation method. They're about three to four months old now.

https://lh3.googleusercontent.com/-o...0/100_0726.jpg https://lh6.googleusercontent.com/-D...0/100_0727.jpg

https://lh3.googleusercontent.com/-p...0/100_0728.jpg

Well. From the above to this:

https://lh6.googleusercontent.com/-4...o/100_1009.JPG

The one in the middle towards the front and the one just to the right in the front are two of the Bulbophyllum orectopetalum seedlings pictured in my previous post. The non-bulbo seedlings pictured are a super-secret primary hybrid which I've grown on for the person doing the breeding.

All of the seed sterilisation and replating was done using Roby's method. It's been amazing and I can't wait to do more.