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I used the same technique, bleach fume ! On 4 flask, only one got contaminated and 2 dried up over time. It's the only flask surviving so far.... It,s a very long process but soooooooo fun to do ! |
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I find that using a glove box with this technique minimize the contamination... but you don't have to... I have use the water steam ( on a wok ) instead of glove box with good results..
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DO you guys think this would work for doing a stem prop? Any thoughts on that?
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Today I try gaseous sterilization.
I used seeds Epipactis helleborine collected in November 2012. Pad was soaked in 5% NaOCl – 3 hours. Seeds were sown on modified BM-2 (with 25% coconut water and 10 grams - not 20 - sucrose per liter). All jars have a lid with a cotton plug. Medium sterilized for one hour in 180C, electric oven. I made 6 jars and now I'm waiting for contaminations ;) Pictures |
Your photos are not appearing. Unfortunately you are not allowed to post photos or links until you have made 5 posts. Post a few more times, and then it's good!
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It's explain why I have a small problems with sharing the photos, thank you ;)
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After ten days I have 8 jars with bacterial contamination...
In two jars I dont see any signs of contamination. I don't know what I did wrong. I have a suspicion: Probably under the orchid seeds could be a bacterial spores, and Cl2 can't kill them, because a part of the surface seed coat didn't avaliable to sterilization agent. |
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Epipactis helleborine have a bigger seeds than other orchids, so I think it's a cause.
Maybe first sterilization seeds in 70% ethanol could solve this problem. I must try it in this weekend. |
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