Micropropagation of Orchid, my final year project

[John D.]

What Royal said is what I do. 10% chlorox for 10 min. This has less chance of killing the seed. I would open the pod and treat the seed you plan to sow, you can keep the rest for another attempt later if you want.

[Bell_bella]

I don't quite get it..Use the normal strength 6% bleach to make a mixture of at a ratio of 1:10 (10% by volume). Dis u mean to use 10% of v/v of bleach? and for how long? 30 minutes should be enough right.

tQ ^^

[Bell_bella]

I think I'm going to propagate it =)

[John D.]

Yes 10% by volume

[Dorothy]

Bell Bella - Since you posted this thread and have gotten responses .. I am going to delete the one I made from your post in the other thread (the one I told you about in the PMs) .. no reason to have duplicate threads
Glad you got some answers

[Bell_bella]

Originally Posted by Dorothy

Bell Bella - Since you posted this thread and have gotten responses .. I am going to delete the one I made from your post in the other thread (the one I told you about in the PMs) .. no reason to have duplicate threads
Glad you got some answers

yeah. I formed this thread b4 u msg me. =) yeah I'm glad too.. thanks

[Bell_bella]

TQ too John D. I'll try 10% v/v and for 10 minutes.

[John]

Did you read the thread "redneck orchid flasking" at the top of this forum? There are a couple of methods mentioned on how to sterilize the seed. I also tried petri dishes and have had too much contamination from the air exchange so I went back to jars. Good luck.
John

[Royal]

Originally Posted by John

Did you read the thread "redneck orchid flasking" at the top of this forum? There are a couple of methods mentioned on how to sterilize the seed.

Why didn't I think of that? Thanks John.

[John]

Royal have you used the petri dishes before? If you have how well did they work? I liked the concept and I still have a few that I made up about six months ago and didn't use for seeds and are not contaminated but overall the ones I used didn't work.
John