Quote:
Originally Posted by SouthPark
That's a really interesting situation, because it was assumed that in general, the mericloning process is supposed to provide results that have exact DNA match. So this leads to some interesting questions about the mericloning process, or cloning process in general.
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In theory it's supposed to yes; but there are so many explants to choose from, techniques to use, culture media, conditions etc that not all clones are created equal.
Mutations will especially happen if the technique uses only a few cells to regenerate hundreds of plants.
Those mutations are called "somaclonal variations"!
I searched for techniques used for Cymbidiums in
Micropropagation of Orchids, Third Edition (Tim Wing Yam, Joseph Arditti) and here are a few of them:
- Shoot meristems cloning
- Culture of floral organs in vitro
- Culture of shoot apices in vitro
- Culture of dormant buds taken from pseudobulbs
- Protoplast isolation from leaves
- Morphogenesis and clonal propagation through
aseptic cell cultures
- Shoot formation from rhizomes
- Plant production through callus culture
If i get my biology right, and if the wanted mutation is stable enough to be passed on from natural division to division, it means the plant has a periclinal mutation.
There is no doubt that this feathering - and all sought after mutations - can be propagated one way or another. It's been widely done in common garden and house plants.