Quote:
Originally Posted by escualida
Thanks Dan, a few more questions: how do you know the right amount of protocorms or seedlings to put in each replated flask?
And for people that don't have glove boxes, how do you make sure you get no contamination when replating?
Also, when replating seedlings, how do you know when it's time for fresh media? How can you tell?
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1. Guesstimate

but it's probably better to go with a smaller amount. You can replate again later if they get too dense.
2. Make a glovebox... it's pretty easy. I can give instructions if you want.
3. Growth slows down and you get some turning brown. I think ppl generally reflask due to overcrowding so may not run into this too often.