How strong does the agar have to be?
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dendrobium agar medium How strong does the agar have to be?
 
 
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Old 01-21-2011, 05:06 PM
Royal Royal is offline
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Join Date: Jan 2008
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Location: Piney Woods of East Texas
Age: 47
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Are you autoclaving the entire batch in one vessel, or dispensing then autoclaving? If the medium doesn't get hot enough to fully dissolve the agar, it gets unevenly dispensed into the flasks. You wind up with a super thick layer at the bottom of the last few flasks and the rest are watery.
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