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  #1  
Old 02-10-2018, 02:36 PM
sweetjblue sweetjblue is offline
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Default Tissue Gard

I'm wondering if anyone on the forum has any experience using the product Tissue Gard.

Saw a listing on Ebay TISSUE GARD Orchid Flask & Tissue Culture Contamination Control | eBay and it states, " revolutionary new preservative manufactured specifically to control contamination in both plant tissue culture and invitro seed sowing and flasking. TISSUE GARD is a heat stable preservative/fungicide that when added at optimal doses, prevents or reduces microbial contamination in plant tissue culture. Because it is heat stable, it can be autoclaved. So it can be added to media before sterilization. It does not interfere with invitro seed germination or callus growth or regeneration"

and additionally it states,

" Nothing was ever added in addition to the TISSUE GARD. Using this method, it would also be possible to flask orchids on the kitchen counter with no flasking box. I have several flasks at different stages of germination all using just the TISSUE GARD with no sterilization done before flasking."

It would be interesting to see if this product actually works as no sterilization would be required and anyone would/could make their own flasks at home without all the hassle that is normally required.

If you've used this product would love to hear from you and how you feel it worked for you


I have no affiliation with the seller or with this product. Just looking for honest info on how this worked for you


.
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  #2  
Old 06-01-2018, 09:05 AM
Dada Dada is offline
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Sounds similar to the Plant Preservative Mixture (PPM).

PPM Store - Plant Preservative Mixture (PPM) - Plant Cell Technology | Fight Contamination With PPM

I have used this particular product and it does work against fungus.

Not sure if you can flask orchids in the kitchen counter without any sterilization whatsoever, but magical things have been known to happen in the past!!
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  #3  
Old 06-01-2018, 01:35 PM
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Ray Ray is offline
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I'd be really interested in knowing what it is, chemically.

Sounds like TGTBT technology.
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  #4  
Old 06-01-2018, 11:56 PM
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estación seca estación seca is offline
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You can't legally sell plant fungicides in the US without stating what is in the package. See if you can find out.
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  #5  
Old 06-02-2018, 08:50 AM
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Quote:
Originally Posted by estación seca View Post
You can't legally sell plant fungicides in the US without stating what is in the package. See if you can find out.
See if you can find out what?

I'm guessing that the individual is buying a larger bottle of something commercial, repackaging-, and renaming it to sell on eBay. The PPM folks state their material is proprietary and have an SDS. I don't see anything about this product.
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  #6  
Old 06-05-2018, 08:33 AM
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Fairorchids Fairorchids is offline
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Totally unnecessary.
  • If you maintain proper procedures when flasking, you should get no (or very little) contamination.
  • If you get contamination in a flask, take it outside into bright sun, and burn the spot away with a magnifying lense (through the wall, don't open the flask). Problem solved.
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  #7  
Old 07-02-2018, 03:46 PM
Polarizeme Polarizeme is offline
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Quote:
Originally Posted by Fairorchids View Post
If you get contamination in a flask, take it outside into bright sun, and burn the spot away with a magnifying lens.
I am so going to try that
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