Water in a Mother Flask
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Old 02-19-2008, 11:01 AM
Royal Royal is offline
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Join Date: Jan 2008
Zone: 8a
Location: Piney Woods of East Texas
Age: 47
Posts: 3,253
Question Water in a Mother Flask

How much water or other liquid overlays is too much in a mother flask? The mother flasks are baby food jars with about 30 ml of medium. There is about 2 mm of sterile, distilled water on top of the seed in one flask. I used more water to get the last of the seed from my test tube, and wound up with about 2 cm of liquid on top of the media in flask #2. They are not vented, so I can't count on evaporation.

Anybody deal with this? What is the most water, disinfectant, nutrient overlay, or other liquid that anybody has been successful with?
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