A seedling story!

[giuseppeorchids]

After some days to the sowing, in two jar started to grow some few mildew, I eliminated them sterilizing whit some few drop of hydrogen peroxide and then removing the mildew spots with the tweezers, after two days in one of the jar there was alredy a bigger mildew spot, so I've alredy removed it using more hydrogen peroxide Now I think it would never re-form

However, the seeds looks like they're becoming green!

[giuseppeorchids]

Ok, maybe hydrogen peroxide is not much good to kill the mildew! today, in both jars there were a lot of new spots of mildew!
So I alredy used roby's gaseous sterilization method, I put a pad soaked with bleach for two hours in both jars, and after a few, the mildew started changing color from blue to brown. No I think that I killed it definitively

According to roby's information, seeds won't die until 4 hours of exposure at bleach fumes, so I think that now seeds could germinate without problems

[Silje]

Originally Posted by giuseppeorchids

Ok, maybe hydrogen peroxide is not much good to kill the mildew! today, in both jars there were a lot of new spots of mildew!
So I alredy used roby's gaseous sterilization method, I put a pad soaked with bleach for two hours in both jars, and after a few, the mildew started changing color from blue to brown. No I think that I killed it definitively

According to roby's information, seeds won't die until 4 hours of exposure at bleach fumes, so I think that now seeds could germinate without problems

This is my experience too. I manage to successfully sow seeds using hydrogen peroxide to keep the flasks clean, but if mold forms at some point during the flasking period, the hydrogen peroxide won't stop it from developing further.

This is also my main challenge with replating... I'm reasonably successful with seeding, but struggle to maintain a sterile environment if I'm forced to open the flasks at some point.

[giuseppeorchids]

Ok, some other jars looks contaminated (in a different way compared to the firts) but now I see the first protocorms in some jars!

[Fairorchids]

Guiseppe,

When you have contamination, you do not have to open the flask. Get hold of a magnifying glass (or the lens from an old pair of binoculars), take the flask outside on a sunny day, and use the lense to concentrate the sun beams onto the spot with mold or bacteria.

This will burn away the problem (and disinfect the spot) through the wall of the flask.

[neophyte]

Maybe the seeds weren't able to germinate?? It looks like you crossed a Dendrobium with a Phalaenopsis. Dendrobiums are pretty different since they are sympodial and Phalaenopsis are actually monopodial. Maybe the DNA was too different? Or maybe the recipe doesn't work on these seeds.

[giuseppeorchids]

Hey there, I'm alive! I'm here for some updates

No, I didn't crossed Dendrobium and Phalaenopsis, I've pollinated a D. lindleyi and some different Phalaenopsis commercial hybrid flowers. The only crossing is a phal flower pollinated with P. equestris!

By the way, this is the situation now: some jars had been contaminated, in others it isn't born anything. Only in 5 jars some seeds have germinated, and most are D. lindleyi
In one jar it is born only one Phalaenopsis!
However, I'm happy for this little result

P.S. in the hydrogen peroxide-treated jars I've killed the mildews, but sadly I've killed also the seeds. They didn't germinated.