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12-02-2014, 08:55 PM
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Senior Member
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Join Date: Jul 2012
Zone: 8a
Location: Athens, Georgia, USA
Posts: 3,208
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Not sure what "Bgltin" is? Gelatin?
My only experience is with the Dokmai Dogma recipe. It does not meet your criteria of not needing to replate, however. When I have used it, when the protocorms seem large enough to survive, I have moved them on to heat-sterilized and slightly damp sphagnum. The seedlings grow very slowly but do eventually grow roots.
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12-03-2014, 02:24 AM
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Senior Member
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Join Date: Jun 2011
Location: In the middle of nowhere - Namibia
Posts: 668
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After trying several home-made recipes I caved in and decided to stick to Sigma P-6668. So far so good without replating. My seedlings have roots that grow nicely into the growth medium.
I'm sowing the laziest way without a glove box, but by using hydrogen peroxide as sterilizing liquid in the flask, and I manage to get germination on the P6668, but struggled with home-made recipes.
Nevertheless, I think all kinds of experiments are good and we need to try to tweak it to fit our needs and resources, so best of luck to you! And your protocorms.
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12-03-2014, 03:30 AM
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Senior Member
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Join Date: Nov 2014
Posts: 161
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yes thank you for the note
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12-11-2014, 01:27 PM
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Senior Member
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Join Date: Nov 2014
Posts: 161
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Bump😁
I will be happy to receive more replies. 😊
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12-17-2014, 01:19 AM
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Senior Member
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Join Date: Nov 2013
Location: Pahoa, Hawai'i, So. Sandwich Isls.
Posts: 537
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Long ago when I used pots quite a bit, I concocted a series NZ sphagnum moss and pumice stone mixes.
The idea being that neither would break down nearly as fast as the bark mixes do.
It seemed to work very well for me.
I have since converted over to purely moss, and don't use pots much at all anymore.
Pumice holds some water and the moss holds a lot of water.
The ratio of moss to pumice and the size of the pumice need to be tailored to the needs of the orchid.
It takes a bit of experimentation to get it right for each orchid's needs.
While I do not remember exactly what ratios I used for what, it seems to me that most varied between 20% to 40% moss to pumice by volume. But, NO GUARANTEES!
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12-20-2014, 01:20 PM
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Jr. Member
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Join Date: Nov 2014
Posts: 17
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Roey, have you tried looking at the posts from the Yahoo group of Home Tissue Culture? Perhaps there is some information there that can help you.
Also, I have used some of Phytotechnology's pre-mixed media for other groups of plants. I don't know how their costs compare with Sigma, but worth a look.
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01-01-2015, 10:55 AM
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Senior Member
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Join Date: Aug 2013
Zone: 7a
Location: North Plainfield, NJ
Posts: 2,819
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If you sow VERY thin, protocorns can grow to maturity in the original flask. But, there will be significant waste from clumps, where protocorns/seedlings crowd each other to death.
If you flask on the kitchen counter, there will be some flasks with contamination. Once you see a mold develop in side a flask, use a magnifying glass as a lense, and concentrate sun beams on the mold. This way you can burn it away without opening the flask, and save the remainder of the flask.
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Post Thanks / Like - 2 Likes
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01-01-2015, 02:00 PM
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Senior Member
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Join Date: Nov 2014
Posts: 161
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Thank you very much fairorchid, but i guess I'm afraid of smashing the small protocons I'm very accurate and sensitive but in picture they look just like little guppies fish which if you hold with something beside a net will be mashed or cut. So are protocons actually more hard😊?
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01-09-2015, 06:14 PM
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Senior Member
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Join Date: Nov 2014
Posts: 161
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Thank you everyone.
Cross we fingers so I will get some seedlings
I will update any month if successful.
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media, orchid, seed, sowing, stage, recipe, doent, require, replating, homemade, 100% |
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