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  #11  
Old 11-07-2014, 10:18 AM
JMLand JMLand is offline
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Quote:
Originally Posted by czayta View Post
Wow, thats awesome!

What % H2O2? I sterlized with 5% Hydrogen peroxide last time I flasked (this past weekend), but that was after a bleach sterlization for 10 mins lol (So far no contamination, but its only been 3 days). How long did you let your seeds sit with the hydrogen peroxide?

How many flasks did you flask and have you had any contamination yet?
I used a 10% solution of peroxide and let it sit for 20 minutes(shaking it around every so often).

I've sown 10 flask total. 5 at a time and had contamination in one. I may have had contamination in another but i treated it by scooping out what may have been infected and using peroxide on the area i scooped out from.

I use a fish tank with holes in the front so my set up isn't the best. The contamination seems to be limited to the last flasks I sow. I think the contamination is from air movement.
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  #12  
Old 11-09-2014, 02:16 PM
Polarizeme Polarizeme is offline
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This is so cool What are you using for a nutrient agar?
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  #13  
Old 11-17-2014, 04:58 PM
JMLand JMLand is offline
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Photo Update on the flasks. I'm going to have ALLOT of plants lol.
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Aerides Houlletiana seeds from ebay-20141112_171924-jpg  
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  #14  
Old 11-17-2014, 04:59 PM
JMLand JMLand is offline
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Originally Posted by Polarizeme View Post
This is so cool What are you using for a nutrient agar?
I use p723 from phytotech. Very similar to p668
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  #15  
Old 11-17-2014, 06:47 PM
BriDroid BriDroid is offline
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That's very cool! Congratulations!
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  #16  
Old 11-17-2014, 07:51 PM
JMLand JMLand is offline
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Originally Posted by BriDroid View Post
That's very cool! Congratulations!
Thanks. I had tried flasking a few years ago and it was a total failure. I decided to give it another go recently and now i have protocorms from 2 species orchids and one of my own hybrids i did just for fun.
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  #17  
Old 11-18-2014, 01:42 PM
Cntry Cntry is offline
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This is great. I would like to know what size fish tank did you use and did you feel you had enough room inside working. I tried using a plastic storage box as a glove box but its just to limited in seeing inside. It fogs up so you can't see what your doing sometimes.
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  #18  
Old 11-19-2014, 11:27 AM
jimbo0108 jimbo0108 is offline
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Hello, I am new to this forum and was curious about a few things about orchid seed sowing and germination. I am currently in the middle of trying to grow from seed...I have done the research on techniques on how to sterilize and sow orchid seeds. I watched a video on YouTube on how to sterilize seeds...The video I found most informative was how to sterilize terrestrial orchid seeds... would this technique work for all kinds of orchid seeds? Also about how long after sowing do they usually germinate?
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  #19  
Old 11-22-2014, 12:13 AM
JMLand JMLand is offline
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Originally Posted by Cntry View Post
This is great. I would like to know what size fish tank did you use and did you feel you had enough room inside working. I tried using a plastic storage box as a glove box but its just to limited in seeing inside. It fogs up so you can't see what your doing sometimes.
I use a 40 gallon that i bought during one of petcos dollar per gallon sale. for the front i use corrugated plastic wit holes cut out. to cover the holes i use rubber gloves cut into a square with a hole cut out and taped to the plastic.

---------- Post added at 11:13 PM ---------- Previous post was at 11:10 PM ----------

Quote:
Originally Posted by jimbo0108 View Post
Hello, I am new to this forum and was curious about a few things about orchid seed sowing and germination. I am currently in the middle of trying to grow from seed...I have done the research on techniques on how to sterilize and sow orchid seeds. I watched a video on YouTube on how to sterilize seeds...The video I found most informative was how to sterilize terrestrial orchid seeds... would this technique work for all kinds of orchid seeds? Also about how long after sowing do they usually germinate?
The sterilization should be the same as far as i know. As for how long until they germinate, it depends on the plants you are working with and the method you are using.

Greenpod method as i understand can take longer than from dry seed.
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  #20  
Old 11-22-2014, 10:51 AM
czayta czayta is offline
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Quote:
Originally Posted by JMLand View Post
I use a 40 gallon that i bought during one of petcos dollar per gallon sale. for the front i use corrugated plastic wit holes cut out. to cover the holes i use rubber gloves cut into a square with a hole cut out and taped to the plastic.

---------- Post added at 11:13 PM ---------- Previous post was at 11:10 PM ----------



The sterilization should be the same as far as i know. As for how long until they germinate, it depends on the plants you are working with and the method you are using.

Greenpod method as i understand can take longer than from dry seed.
in my experience that is correct. My Greenpod took two and a half months to germinate and various dry seeds have taken only one month to germinate.
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