GERMIANTION MEDIA STERILSATION PROBLEMS

[ferramont]

Hello dear orchid enthusiasts,

I am doing my first testings in preparation of the germination media. I don't have a pressure cooker, so I sterilise in the microwave oven. once the media is prepared, (the banana and tomato recipe) with the agar, it all starts to become a gelly correctly as it cools down. but then, when I sterilise at the microwave, it all boils again. All flasks are put to maximum power for 3 times 1 minuts exposure. So after this process it all turns liquid again and the resulting gel is not as good. Any ideas? any correction to do?
thanks!

[OCRC_Dir_China]

After you heat it to a gentle boil, and load you vessel/flask don’t let it cool, take it to the microwave sterilize it then let it cool down, before you sow your seeds on to it.

[shushu45]

I read somwhere that when making ur own media u sould seal them before they cool down and wait a few days to make sure they aren't contaminated.

[OCRC_Dir_China]

Some people do this; at the OCRC we don’t practices this, attention to detail makes a lot of difference.