Sterile dry seeds methods

[roby]

Hi NatalieS

It seems that even Milton's is based poclorito sodium 2%, while the 5% chlorine bleach.
So probably with Milton you can lengthen the exposure time and less likely to die seeds.
thanks

[Larssh]

Originally Posted by zxyqu

The video Natalie posted is an excellent source and works quite well. One note of caution, is that some seeds are more delicate than others, and bleach will be an issue. You can use hydrogen peroxide as well for those that might be a little more "needy." I normally do 1/2 in hydrogen peroxide/soap solution, and the other 1/2 in bleach/soap solution, and follow the videos steps after that. Works like a charm, and ensures I dont lose all my seed.

How strong a solution of hydrogen peroxide do you normaly use, and what kind of soap do you mix it with and how long ?
Sorry about all those question.
regards Lars

[zxyqu]

Just general hydrogen peroxide you can buy at the store, and any general dish soap works for me. It's exactly what he does, but the addition of some soap really helps wash some of the crap off.

[Bloomin_Aussie]

Not just that... it acts as a wetting agent to stop the seed floating which helps the disinfectant do its job.

[Larssh]

Thanks both of you, mystery solved ;-) Iam trying to suck as much knowledge to me as posible.

[netpumber]

Originally Posted by zxyqu

The video Natalie posted is an excellent source and works quite well. One note of caution, is that some seeds are more delicate than others, and bleach will be an issue. You can use hydrogen peroxide as well for those that might be a little more "needy." I normally do 1/2 in hydrogen peroxide/soap solution, and the other 1/2 in bleach/soap solution, and follow the videos steps after that. Works like a charm, and ensures I dont lose all my seed.

I have two kind of seeds.
1) Phalaenopsis
2) Anacamptis pyramidalis

Can you say in which one to use the hydrogen peroxide and in which one to use bleach ?

Also tomorrow i ll sow these seeds and thought to use both of disinfection methods(syringe & vapor disinfection).

zxyqu you only use syringe method ?

[zxyqu]

I have no idea about the second one, but both 10% bleach and H2O2 should work for phals. I only sterilize the seed with the syringe technique. But I sterilize the bottles/media via autoclave or pressure cooker.

[netpumber]

Ok Thank you. I will only use 10% bleach because last year i tried it with an other terrestrial genera (Dactylohriza saccifera) and they got germinated.

[netpumber]

zxyqu what proportion in bleach you use?

[zxyqu]

What do you mean net? I use a 10% solution, though I admit I always try to be a little less than actually 10% (probably more like 9-10%)