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  #11  
Old 10-03-2007, 02:31 PM
John John is offline
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Location: Rockford, IL
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Hello Jean Pierre,

I agree with Nguyen..WOW! I bet you can flask in your sleep. A sterile room is not going to happen for me. The best I will get is the laminar flow cabinet. I also probably will do only 20 to 30 flasks/replates a year. If I do not vent the jars, then I can take the foil off and spray them well with bleach. (I have been using the bandaid over a hole in the lid method and didn't want to get the bandaid wet.) I look forward to seeing your pictures.

John
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  #12  
Old 10-04-2007, 03:15 AM
adriponic adriponic is offline
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hi here are the pics[IMG]c:\Documents and Settings\adrien\My Documents\LABO1.htm[/IMG]

ooops they wont upload - plse can i have your email, John and I'll forward the pics. You might then
post them in this thread.

Last edited by adriponic; 10-04-2007 at 03:17 AM.. Reason: pics would not upload
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  #13  
Old 10-04-2007, 02:27 PM
John John is offline
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Hi Jean Pierre,

Is it possible for you to modify your profile and add your email address? I can then contact you that way. The other idea would be if someone out there could help with the picture upload.

John
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  #14  
Old 10-04-2007, 05:19 PM
adriponic adriponic is offline
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ok john

my contact - adriponic@yahoo.com
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  #15  
Old 10-05-2007, 12:00 PM
John John is offline
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Laminar flow cabinet Male
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Hi Jean Pierre...I have resized your pictures and I hope they come through ok. I was having trouble with some of them and I don't know why. There also is a limit of 10 to upload. You should be proud of what you have acomplished. It looks like you have put a lot of thought into this operation. Sure could use some of those tomatoes.

John
Attached Images
File Type: jpg autoclave2.jpg (13.2 KB, 224 views)
File Type: jpg autoclave.jpg (14.2 KB, 159 views)
File Type: jpg cucumber.jpg (27.9 KB, 187 views)
File Type: jpg flask.jpg (11.0 KB, 197 views)
File Type: jpg laminarflow.jpg (13.8 KB, 269 views)
File Type: jpg letuce.jpg (33.6 KB, 150 views)
File Type: jpg Media prep room.jpg (15.3 KB, 203 views)
File Type: jpg sterile room.jpg (13.3 KB, 221 views)
File Type: jpg protocorms2.jpg (29.4 KB, 192 views)
File Type: jpg protocorms.jpg (12.0 KB, 183 views)
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  #16  
Old 10-05-2007, 01:06 PM
adriponic adriponic is offline
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wow John

You did a nice job! I dont know how to resize jpegs. In fact there are two things I hate much - 1. Paint works and 2. photography. Thanks for uploading the pics. If you need some more info that I can help please just ask.

Btw, breeding tomatoes is a lot quicker than breeding
orchids, but the end result is the same... once in many
thousands the progeny comes out with marked
enhancement over the parents.

Happy sowing, John
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  #17  
Old 10-05-2007, 01:30 PM
newflasker newflasker is offline
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Hi Jean,
I love your pictures especially lettuce, preparation room, and picture of your son holding tomatoes. You sowing tomatoes using seeds or tissue culture?
Nguyen
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  #18  
Old 10-13-2007, 09:31 AM
Charles Charles is offline
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Great job guys. I hope I can do this flasking thing. But I now feel like Fred Flintstone. I'm going to try this for the first time by beating the snot out of a dinosaur with a big wooden club. At least that is how I feel about my technology.
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  #19  
Old 10-13-2007, 10:11 PM
John John is offline
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Hi Charles

Thank you for jumping in. Good to hear that you are going to try flasking. Tell me about what you have assembled and how you are going to do the flasking. I am always looking for more info. When I started this thread, I hoped to make a low cost laminar flow cabinet. To that end I have ordered some parts and have a plan in mind. The cost will be about $400, more than I first thought. The next step will be assembly and testing followed by learning more about media and technique. I think a lot of the fun is in doing the research and putting it all together. Look forward to hearing from you.

John
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  #20  
Old 10-14-2007, 08:40 AM
Charles Charles is offline
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John,
Like I said. I am going to beat the snot out of a dinosaur with a big wooden club and wham! Flasking.

I have been studying techniques myself. I will not have a hood or a sterile room. I will probably be working in a box with rubber gloves duct taped to the holes. And a big wooden club. Just in case of dinosaurs.
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