My attempt at flasking!-vapor sterilization

[camille1585]

Originally Posted by Wynn Dee13

Bob- Do you use the same method to replate them too?
Can you tell me where I could find the thread that you learned this technique from? Thank you!

Here: http://www.orchidboard.com/community...y-seeding.html
Roby modified/improved an existing technique for gaseous disinfection of orchid seed, and adapted it for sterilizing the entire flask.

[Wynn Dee13]

Thanks Camille!

[roby]

Originally Posted by Call_Me_Bob

thanks for posting it originally! im so glad i found it and i hope i have as much success as you did!

learn, without divulging what you know has no meaning, and write in this forum has greatly improved the method


@ jaxorchidman very well!!!!

[Call_Me_Bob]

Originally Posted by Wynn Dee13

Bob- Do you use the same method to replate them too?
Can you tell me where I could find the thread that you learned this technique from? Thank you!

i have no idea about replating... dang, i shouldbe thought about that!!!!

roby? what should i do?

Originally Posted by camille1585

Here: http://www.orchidboard.com/community...y-seeding.html
Roby modified/improved an existing technique for gaseous disinfection of orchid seed, and adapted it for sterilizing the entire flask.

thanks camille!

Originally Posted by roby

learn, without divulging what you know has no meaning, and write in this forum has greatly improved the method


@ jaxorchidman very well!!!!

thanks for sharing it in the first place roby!

[roby]

Originally Posted by Call_Me_Bob

i have no idea about replating... dang, i shouldbe thought about that!!!!

roby? what should i do?

yes sure you can use, I do.
Maximum time of exposure, 2 hours and 30 minutes.
But it is important to do this in glow box, to limit contamination

[Call_Me_Bob]

Thank roby!

Why should it be done in a glove box if the fumes are still supposed to sterilize it?

[FairyInTheFlowers]

Originally Posted by Call_Me_Bob

Thank roby!

Why should it be done in a glove box if the fumes are still supposed to sterilize it?

I would assume its so that there is a minimum of microbes to get into the medium that have to killed with the fumes.

[roby]

Exact, in the case of sowing 5 hours, enough to kill any contamination, but in these cases, 2.30 hours, may not be enough to kill any contamination.
More time, unfortunately damage to plants.

[camille1585]

Any signs of germination or contamination Bob? I'm curious!

[Call_Me_Bob]

Originally Posted by camille1585

Any signs of germination or contamination Bob? I'm curious!

there has been contamination in a few of the "experimental ones"-the ones that i used tubberwear food storage containers. all of the jars have not contaminated hopefully they wont. no germination yet, i think its a bit too early for that