Contamination problems

[Mxptrsn]

Originally Posted by Tsuchibuta

I haven't really had a significant amount of post sow contamination, in any method that I try (glovebox, open air, and gas fumigation). It could be because I'm very methodical about making sure everything has been sterilized that I'm using. I will wipe the outsides of containers with lysol or bleach prior to opening any of them. Personally I don't find ethanol to be a very viable sterilization method (in 70% strength). When I used to do volunteer work in the herpetology dept. at a natural history museum, I was told that we had to use 80% or stronger in the specimen jars. I have tried lysol brand wipes and they seem to be very effective (and smell nice too) at sterilization.

Ultimately I think it's key to work fast. The less time the flask is open the less chance of contamination.

Today, I've tried everything you said. And, you're absolutely right. The result is really satisfying. Thank you!

Have a great and successful life, Tsuchibuta.