Very Easy Sterilisation Method - My Seedlings

[camille1585]

I have never heard of that method of sterilization before, but it sounds worth a try! I'm very very surprised that you only get 10% contamination with this, that's amazing. I have some seed to flask, and I think I may try to flask some with this method, it sounds much easier than the 'normal' way of doing it.

Do you sterilize the the medium beforehand (pressure cooker for example?) Do you disinfect the seeds before or is the bleach enough to do it?

And would you mind sharing your homemade medium recipe?

Don't worry about your English, it's quite good and I had no trouble understanding!

[roby]

Originally Posted by camille1585

Do you sterilize the the medium beforehand (pressure cooker for example?)

oven (150 ° C 25 minutes)

not disinfect the seeds, in fact if you go over 5 hours die fungi, bacteria and seeds

my medium is simple:

1 L RO water
1.5 g 20-20-20 NPK (Peters formula)
20 g sugar
1 g activated charcoal
5 drops NAA
5 g Agar

PH 5.8

This medium does not work with Catleya, aerangis, spathoglottis

has been tested with
Bletilla
Phalaenopsis
Doritis
Dendrobium
Sophronitis
Restrepia
Masdevallia


I'm happy if you try

There are few rules
clean all tools
do not put head and hands over the jar
prepare the absorbent pad on a blotting paper soaked in alcohol to prevent the electrostatic action of plastic. (picture 1 and 2)

I'd like to know the results

[camille1585]

Thanks! I just need to find the NAA, otherwise I have everything else. I have Cattleya, Brassavola and Dendrobium seed, so I guess I'll try to make a flask of Den seeds. But I'm in the netherlands and my seeds are in france, so it will have to wait a few months.

[dendro king]

Originally Posted by roby

oven (150 ° C 25 minutes)

not disinfect the seeds, in fact if you go over 5 hours die fungi, bacteria and seeds

my medium is simple:

1 L RO water
1.5 g 20-20-20 NPK (Peters formula)
20 g sugar
1 g activated charcoal
5 drops NAA
5 g Agar

PH 5.8

This medium does not work with Catleya, aerangis, spathoglottis

has been tested with
Bletilla
Phalaenopsis
Doritis
Dendrobium
Sophronitis
Restrepia
Masdevallia


I'm happy if you try

There are few rules
clean all tools
do not put head and hands over the jar
prepare the absorbent pad on a blotting paper soaked in alcohol to prevent the electrostatic action of plastic. (picture 1 and 2)

I'd like to know the results

Hey guys, would anyone be able to tell me what NAA would mean. Thanks

[orchiddream]

can I do this with replating as my seeds arnt growing and lots of condensation in jars, they are approx. 1mm and I did them in November.

[OCRC_Dir_China]

Are you replating in a seald jar or flask?

[orchiddream]

a glass jar

[OCRC_Dir_China]

You can try cotton wool plug in the lid or tape some cotton wool over the hole. But you need to leave a small part untapped over the hole, so the jar can breathe.

[Catmarak]

I have used this method with Oeceoclades, Eulophia, Cynorkis, Ansellia species and Cattleya hybrids. I am so glad I found this.