Very Easy Sterilisation Method - My Seedlings

[roby]

Originally Posted by Fernando

Thats amazing! I've just sowed the traditional way in a glove box, next time I'm trying this method!
Thanks!!! It will be great for replating!

Fer

for replanting, no more than 2.30 hours
avoid browning of some leaves, and the plants will start soon

---------- Post added at 08:55 AM ---------- Previous post was at 08:50 AM ----------

Originally Posted by CymPaphZygo

Very intruiging - i was wondering if you have deflasked any of the plantlets

only a Bletilla ochracea (November) , I left the small bulbs in the fridge until last week, and now is in pots outside, germinates in the spring...I hope

[daniel]

good post Roby

i will try this also
to bad i did not see this post before
i have been doing some den seeds last weekend
if i new this sooner.i could give it a try :-(

but i have a question on your post in the past on your
Pollinate tips
i have been having good success on hand pollinate on all my orchid flower
but there one that will not work
Psychopsis Papilion?

[Fernando]

Just found five flasks with contamination.
I tried this bleach fumes technique, exposing the five flask 30 minutes to fumes. We'll see if it works. If it does, I've a lot of photos to post!
Regards
Fer

[Phalguy]

[quote=roby;463985]for replanting, no more than 2.30 hours
avoid browning of some leaves, and the plants will start soon

---------- Post added at 08:55 AM ---------- Previous post was at 08:50 AM ----------



Do you mean that we can use the fume technique for replating?

[roby]

Just found five flasks with contamination.
I tried this bleach fumes technique, exposing the five flask 30 minutes to fumes. We'll see if it works. If it does, I've a lot of photos to post!
Regards
Fer

too little 30 minutes, if they are seeds you can get to 4 5 hours if seedlings are 2 to 2.5 hours

Do you mean that we can use the fume technique for replating?

Of course, even better than for sowing, in the transplant it only has to sterilize any surface contamination.

transplants never had no contamination, this allowing me to make transplants more frequently

[Tsuchibuta]

Originally Posted by Fernando

Just found five flasks with contamination.
I tried this bleach fumes technique, exposing the five flask 30 minutes to fumes. We'll see if it works. If it does, I've a lot of photos to post!
Regards
Fer

So does this technique actually work to decontaminate the flasks as well?

[roby]

Originally Posted by Tsuchibuta

So does this technique actually work to decontaminate the flasks as well?

never tried it on contaminated flasks, but if the contamination is not extensive, you can try to replant and use this technique

[NatalieS]

I am going to try this method this weekend with bellatulum dendrobium seeds. It will be my first time attempting to germinate orchid seeds so I'm actually not expecting it to work, but here's hoping!

[roby]

can improve the system, using the glow box as indicated by Phalguy

[NatalieS]

Well, last Saturday I made up my first small batch of flasks. I used the microwave method to sterilise the medium, and Roby's method to sterilise the seed.

It will be a week tomorrow and I don't see signs of contamination yet. I don't see anything else happening either. I'm guessing it will be a month or so before I'll know if the seed is going to germinate? I used dendrobium bellatulum seeds - does anyone know how long they take to germinate?