Very Easy Sterilisation Method - My Seedlings

[Juan Aguiar]

Originally Posted by Dorchid

**** chlorine gas. (Redacted by Moderator) If you don’t have access to a flow hood you can work inside a still air box made from a Rubbermaid tub. Additionally, using plant protection mix in your media is super effective at preventing contamination, so much so that it’s reported that plating can be done in open air.

What would that phytosanitary mixture be about in the culture media? Is there a topic in the forum where it is explained?

[Dorchid]

It may have been discussed somewhere on here before but I haven't looked. It's a media addendum that helps prevent contamination by fungi and bacteria and can also be used to surface sterilize seeds and explants if the typical protocols using bleach, etc. aren't working. A little bit goes a long way. Here's the website, check the product description and FAQs. Plant Preservative Mixture (PPM) 30 Milliliters

[Catmarak]

I have used this method with Oeceoclades, Eulophia, Cynorkis, Ansellia species and Cattleya hybrids. I am so glad I found this.