Very Easy Sterilisation Method - My Seedlings

[omega_z]

interestibg method. are other types of sterilization agent can be used? such as alcohol, hydrogen peroxide? or it just the one which has volatile properties. thank you.
regards,
yanto

[roby]

only gaseous agents such as chlorine, can perform this type of sterilization

---------- Post added at 06:18 AM ---------- Previous post was at 06:13 AM ----------

Originally Posted by biofuel

Epipactis helleborine have a bigger seeds than other orchids, so I think it's a cause.
Maybe first sterilization seeds in 70% ethanol could solve this problem.
I must try it in this weekend.

sowing with very few seeds, I think there are too many big pieces

[Rinzo]

Just want to report that i had my first success using this method on the first try! ill post pictures along with details later on once the seedlings are a little more visible since i just was just able to see germination today 8/24/13.

[dendro king]

Originally Posted by roby

oven (150 ° C 25 minutes)

not disinfect the seeds, in fact if you go over 5 hours die fungi, bacteria and seeds

my medium is simple:

1 L RO water
1.5 g 20-20-20 NPK (Peters formula)
20 g sugar
1 g activated charcoal
5 drops NAA
5 g Agar

PH 5.8

This medium does not work with Catleya, aerangis, spathoglottis

has been tested with
Bletilla
Phalaenopsis
Doritis
Dendrobium
Sophronitis
Restrepia
Masdevallia


I'm happy if you try

There are few rules
clean all tools
do not put head and hands over the jar
prepare the absorbent pad on a blotting paper soaked in alcohol to prevent the electrostatic action of plastic. (picture 1 and 2)

I'd like to know the results

Hey guys, would anyone be able to tell me what NAA would mean. Thanks

[roby]

is an Auxin
1-Naphthaleneacetic acid - Wikipedia, the free encyclopedia

[Orchid Whisperer]

I am planning to flask some Cattleya seeds this week.

I have some jars ready that I plan to sew using more conventional methods, but I also want to try this method; I have some extra medium left over, and of course, plenty of seeds.

My medium is home-made, but a different recipe. Google "How to grow orchids from seeds - kitchen style", you will find it.

roby - thank you so much for posting this method!

[alaind52]

Hi Roby,
I am new in the "In vitro" sowing and I find the classical method a bit complicated. I have some jars ready for 3 weeks and, so, would like to try the way of sterilization you talked about. I have a solution of sodium hypochl. at 3,6% (12° Chl.). Do you think that the concentration is enough? What level of concentration do you use? Thanks a lot,

[Rinzo]

[frozzybear]

[/COLOR]

Originally Posted by Phalguy

Hello Jeff9

I do pollinate and harvest seeds from my phals...
After a few try, I have better chance when:
1. I cross an old flower on a young flower ( same thing as humain no? ;-) )
2. pollinate in the morning
3. Don't remove the pollen on the mother flower
4. If selfing, flowers should be on 2 different spike
5. Orchid can be very stubborn!

Since I do that, I have almost no fail...

Adding my two-cents to the above, which is a GREAT guideline...

If dealing with fragrant orchids (such as Catt's) wait to do pollinating/harvesting of pollen until the flower in question is fragrant (I've had luck with warm days, mid-morning when I begin to notice strong fragrance... skip overcast/cool days)
I'm supposing the plant knows when it is most fertile and sends out the fragrance to attract pollinators.
Also (I would imagine) that nighttime fragrant orchids may be best pollinated at night.

-LVD

Use what talent you possess - the woods would be very silent if no birds sang except those that sang best.
Henry Van Dyke

[frozzybear]

Just completed a series of replates using the gaseous sterilization method described above (with bleach soaked cotton balls for pads, without the use of a hood)... *fingers crossed*

A thought I had while replating:
It seems beneficial to mix a bit less water into the usual agar/nutrient solution so that it firms-up more than usual in the autoclave process.

This should cut down on the amount of plant material that sinks into the agar during replating, thus increasing the amount of plant material exposed to gas exchange for sterilization.

Another wards - a replate plantlet sitting on top of a firm agar surface won't sink.

Flask seedlings being replated:
C. Jose Marti 'Mother's Favorite' x self
This seemed like a perfect opportunity to experiment with this new technique since the flask had around 100 mini seedlings.

(I read about the Jose Marti and Mother's Favorite both being a registered cross of the same parents but registered by different people while the parents had different names, boy oh boy does the naming get confusing!)

-Laura

Use what talent you possess - the woods would be very silent if no birds sang except those that sang best.
Henry Van Dyke