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  #1  
Old 01-26-2011, 06:33 PM
Mcfacker Mcfacker is offline
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Something's wrong in my Flask! - Help
Default Something's wrong in my Flask! - Help

Hi
Fairly stil a newbie here but am getting better.
I bottled some Dendrobium Seeds and they germinated about 4 weeks later, now in their 6th week, I am begining to see some white creamy susbtance around the protocorms, if I may call them this.

I have had this same problem in one other bottle. From what I gather the creamy susbstance formed mostly where there was initially liquid/water in the jar that seems to have been used up the protocorms. The protocorms on the cream seem to be doing OK, but just as a precaution I have removed some out from uncontaminated areas and platted them in another bottle.

I have attached 2 Pixz so that someone might be able to identify this. I have also, uploaded 2 of the ones that aren't contaminated. I have used a Fertilizer, multi-vitamin mix with Honey and agar. Honey seems to stimulate growth better than sugar.

My appologies for the poorly shot images.
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Something's wrong in my Flask! - Help-dsc03661-jpg   Something's wrong in my Flask! - Help-dsc03660-jpg   Something's wrong in my Flask! - Help-dsc03655-jpg   Something's wrong in my Flask! - Help-dsc03662-jpg  
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  #2  
Old 01-27-2011, 11:00 AM
Royal Royal is offline
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It's hard to tell from the pictures, but it sounds like contamination. Since it appears around each protocorm I would guess that it's from the seed. You might need to step up your seed sterilization (duration or concentration). How did you sterilize the seed?
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  #3  
Old 01-27-2011, 08:38 PM
Mcfacker Mcfacker is offline
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Something's wrong in my Flask! - Help
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Thanxz Royal
I used the simplest guide available to me at the time. Green pods were used. guide is Simple orchid flasking | Flowers Growing

The white substance is concentrated around where water stood earlier that's now been absorbed by the protocorms. Have you got any ideas on if this could be the fungal contaminant that actually aids germination! Read this somewhere but blank about it. The protocorms are surviving for now, wil update again later.
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  #4  
Old 01-30-2011, 06:02 PM
Mcfacker Mcfacker is offline
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Update,
The protocrms don't seem to be doing all that well, I want to do a replate to try and save some. Can someone pliz tell me what I could use to disinfect these plus the concentration would be helpful.
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  #5  
Old 01-30-2011, 08:54 PM
newflasker newflasker is offline
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Something's wrong in my Flask! - Help
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When you use fertilizer to make your media you need to choose a fertilizer which has all macro and micro elements such as N, P, K, Ca, Mg, S, B, Mo, Fe, Cu, Zn.... Missing one of them your seedlings don't grow real well. Some fertilizers don't have Ca, Mg and some cheap fertilizers don't have micro elements. Use strong amount fertilizer for sowing and half strong amount for replating.

Last edited by newflasker; 01-30-2011 at 08:56 PM..
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  #6  
Old 01-30-2011, 09:57 PM
Mcfacker Mcfacker is offline
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Sorry New Flasker, I think u misunderstood my question.
I want to know what I could use to treat the infected protocorms - with minimum damage.
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  #7  
Old 01-31-2011, 10:16 AM
Royal Royal is offline
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Saving them at this stage is hit and miss. Chlorine bleach may work. I'd use a 10% solution.
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