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01-03-2011, 09:23 PM
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Senior Member
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Join Date: Jun 2010
Location: General Trias, Cavite
Posts: 283
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Quote:
Originally Posted by roby
CRH is an hormone, I do not understand
Activated charcoal is present in many professional culture media , is not my idea: D
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hello roby, thanks for the reply. kindly bear with me as i'm new to this and have not really done any flasking but i would like to do something different and would like to experiment on it. my goal is to be able to do it w/o the sterilization steps. that would mean a medium that is sterile in itself. i learned that CRH (carbonated rice hull) is sterile and might be a good sterile medium to use. i don't know if that's a right thinking. do you think that would work? maybe I should mix a few patato, sugar, banana and put it in CRH and see if there will be contamination.
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01-04-2011, 04:26 PM
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Senior Member
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Join Date: Mar 2008
Zone: 8a
Location: Central Texas
Posts: 518
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This is an interesting post. I don't think one can "plate" orchid seed without some form of sterilization. The "bugs" reside in the air, on your skin, on the cabinet tops....there are little spores and what not everywhere, even on dust grains. You may start with a sterile media, but once you have opened it, whether to add the seeds or to place it into the container that will ultimately hold the seeds, presto, it's a pretty safe bet that its contaminated. (Hence laminar flow hoods equipped with uv lights). I had good success adding banana to a charcoal based media that I purchased from Sigma. If you are wanting to go with a more "fundamental" recipe, as in one you come up with yourself, there were a couple of posts with good ones that I might have to try . I have always used agar in my media mix, but I wonder if one could use unflavored gelatin with the same success? I have a B. nodosa pod that is ripening, maybe it's time for another try! Regardless, good luck to you and definitely share your results!!
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01-04-2011, 07:12 PM
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Senior Member
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Join Date: Nov 2010
Location: Naples, IT
Posts: 305
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@ Zildjian patato, sugar, bananas are organic material, then are attacked by fungi and bacteria if you do not sterilize
@ Vanessa you can try
I tried with a paper towel (Sophronitis coccinea) but grow slowly
Last edited by roby; 01-04-2011 at 07:14 PM..
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01-04-2011, 09:39 PM
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Senior Member
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Join Date: Jun 2010
Location: General Trias, Cavite
Posts: 283
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thanks for the reply. will share any results from my wierd idea on doing this.
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01-04-2011, 09:48 PM
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Senior Member
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Join Date: Mar 2008
Zone: 8a
Location: Central Texas
Posts: 518
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roby,
you have inspired me!
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01-26-2011, 06:44 PM
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Jr. Member
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Join Date: Oct 2010
Posts: 18
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Hope this helps, I have used some and been successful, I wouldn't however use Rain water, it is contaminated on the way down to earth and also by the media it runs off of. I have had success with the fertilizer mix, but mine isn't exactly the same as below. I have used what's available in my country. Also, honey instead of sugar is a great stimulant for growth.
Here you have some media ordered by complexity:
BANANA FORMULA
100.0 g of banana
10.0 g of agar agar
15.0 g of sugar
1.2 g Tiamina (Vitamin B1, 4 tablets of 0.3g)
900 ml of distilled water
TOMATO FORMULA
250.0 g of mature tomato
10.0 g of agar agar
15.0 g of sugar
1.2 g Tiamina
900 ml of distilled water
PINEAPPLE FORMULA
200 ml of pineapple juice
10.0 g of agar agar
15.0 g of sugar
1.2 g Tiamina
800 ml of distilled water
DYNAGRO FORMULA
3 cc DynaGro 7-9-5 or 10-5-5
12.0 g of agar agar
20.0 g of sugar
2.0 g of charcoal or activated coal
5 tomates without seeds
150.0 ml of coconut water
40.0 g of banana
800 ml of distilled water
FORMULA TOMATO AND CARROT
100.0 ml of carrot juice
100.0 ml of tomato juice
10.0 g of agar agar
15.0 g of sugar
1.2 g Tiamina
800 ml of distilled water
TOMATO AND BANANA FORMULA
40 g of banana
100.0 ml of tomato juice
10.0 g of agar agar
20.0 g of sugar
1.2 g of Tiamina
900 ml of distilled water
HAWAIIAN FORMULA
1.5g of fish emulsion
1.0g peptona
12.0 g of agar agar
20.0 g of sugar
1 liter of distilled water
FORMULA KNUDSON "C"
1.0 g of Calcium Nitrate
0.250 g of monopotassium Phosphate
0.250 g of Magnesium Sulfate
0.500 g of Ammonium Sulfate
0.025 g of Iron Sulfate
0.0075 g of Manganese Sulfate
20.0 g of sugar
10.0 g of agar agar
1000 ml of distilled water
FORMULA BURGEFF
1.0 g of Calcium Nitrate
0.250 g of Ammonium Sulfate
0.250 g of Magnesium Sulfate
0.020 g of Iron Sulfate
0.250 g of bipotαsic Phosphate
0.250 g of monopotassium Phosphate
20.0 g of sugar or honey
12.0 g of agar agar
1 liter of distilled water
FORMULA THE GARDE
1.0 g of magnesium Sulfate
1.0 g of Calcium Nitrate
1.0 g of monopotassium Phosphate
1.0 g of Calcium Chlorate
0.500 g of Ammonium Nitrate
0.500 g of Ammonium Carbonate
0.050 g of Iron Phosphate
20.0 g of sugar
12.0 g of agar-agar
1 liter of distilled water
FORMULA JUVENAL MEYER
500 ml distilled water.
500 ml of tomato juice
20 gr of sugar, honey, glucose or dextrose
14 gr of agar agar
2 gr of coal activated in powder
1 gr pay 30-10-10
COMERCIAL FORMULA
M and S
Phytamax
FORMULA FOR TERRESTRIAL ORCHIDS
MgSo4 150mg
Kh2Po4 150mg
Ca3Po4 90mg
FeSo4 18mg
Tryptone 3,5-4,0g
Nitsch Vit. 108mg
MONTH 1g
act. charcoal 1g
Agar 5-6g
sucrose 20g
chunks of potato (10 for 50ml of way) of potato cut 5x5x5mm COMMERCIAL
FORMULA FOR TERRESTRIAL
TQPL orchid media
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09-06-2015, 12:38 AM
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Jr. Member
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Join Date: Aug 2015
Posts: 4
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germination to deflasking
I recently de flasked some Daracula Gigas successfully. In under 6 weeks and all I used was an old 200 gram jar for coffee. Washed and rinsed thoroughly. Microwaved for 30 secs.
Sterilised some coconut fibre stuffed it in the jar.
Put in the seeds. Left it sealed for 2 weeks and great success.
P.m. if u want further details.
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09-06-2015, 01:17 PM
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Senior Member
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Join Date: Jan 2008
Location: Espaρa
Posts: 496
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A photograph, please.
Sounds like these seeds from aliexpress are quick growing like weeds ...
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09-16-2015, 02:38 AM
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Jr. Member
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Join Date: Sep 2015
Posts: 1
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Hi everyone, thanks for sharing your wonderful formulas!
I have two questions about flasking medium:
(1) pH level - we've mentioned this more than once here in this thread. So what's the ideal / conducive pH level for seed germination?
(2) Agar viscosity - What makes an ideal viscosity for flasking medium to bed the seeds? From photos they look hard like melted candles. Or are the flasking mediums as thick and viscous like tomato soup?
I'm a newbie here, interested to learn more about flasking.
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10-08-2016, 02:24 PM
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Jr. Member
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Join Date: Oct 2016
Posts: 1
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Please , can you explain what is agar ?
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