Flasked seeds and cells,- NOW WHAT?
Login
User Name
Password   


Registration is FREE. Click to become a member of OrchidBoard community
(You're NOT logged in)

menu menu

Sponsor
Donate Now
and become
Forum Supporter.

Flasked seeds and cells,- NOW WHAT?
Many perks!
<...more...>


Sponsor
 

Google


Fauna Top Sites
Register Flasked seeds and cells,- NOW WHAT? Members Flasked seeds and cells,- NOW WHAT? Flasked seeds and cells,- NOW WHAT? Today's PostsFlasked seeds and cells,- NOW WHAT? Flasked seeds and cells,- NOW WHAT? Flasked seeds and cells,- NOW WHAT?
LOG IN/REGISTER TO CLOSE THIS ADVERTISEMENT
Go Back   Orchid Board - Most Complete Orchid Forum on the web ! > >
 
 
Thread Tools Rate Thread Display Modes
Prev Previous Post   Next Post Next
  #1  
Old 03-11-2010, 08:18 PM
Florida_guy_26 Florida_guy_26 is offline
Senior Member
 

Join Date: Oct 2007
Zone: 8b
Location: NW FL
Posts: 139
Default Flasked seeds and cells,- NOW WHAT?

Ok, So I just today flasked some dendrobium anosmum and den. udom sri seeds. I flasked Cattleya walkeriana var. tipo 'cambara cell samples I took from an older severed back bulb as well. The cells and seeds were placed on agar in my flasking case with the air purifier on so I hope everything was sterile.

I DO NOT have an autoclave/pressure cooker as I just moved and would not know how to use it anyway. For me, micorwaving is the easiest way to do the agar and just do my best to keep contaminants out.

I put everything in the case and sprayed it all with disinfectant including the needles and syringes with cells and the containers full of seeds. But my question now is, what do i do???

I flasked and now do I just cross my fingers and hope that everything is sterile and the seeds are still viable? I am hoping to see some growth soon, but I know it can take anywhere from 2 weeks to 6 weeks for the seeds to START germination and cells might be a bit less. I guess now I have to play the waiting game.

I have the jars inside the flasking case and will keep them there and just use flourescent lights for 8-10 hours a day until i see green in the jars- I hope the good green and not the nasty green. I am keeping my fingers crossed as this will be my last attempt at in-vitro orchid culture- if I do not have luck with the seeds germinating and cells growing, then i give the hell up.
Reply With Quote
 

Bookmarks

Tags
catt walker 'cambara', cells, den. hybrids, flasking, seeds, cells, -, flasked


Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


Similar Threads
Thread Thread Starter Forum Replies Last Post
Choosing Seeds (or How to Navigate the OSP Seed List) Jensine449 Propagation 18 10-04-2009 07:22 PM

All times are GMT -4. The time now is 09:39 PM.

© 2007 OrchidBoard.com
Search Engine Optimisation provided by DragonByte SEO v2.0.37 (Lite) - vBulletin Mods & Addons Copyright © 2025 DragonByte Technologies Ltd.
Feedback Buttons provided by Advanced Post Thanks / Like (Lite) - vBulletin Mods & Addons Copyright © 2025 DragonByte Technologies Ltd.

Clubs vBulletin Plugins by Drive Thru Online, Inc.