Flasked seeds and cells,- NOW WHAT?
Ok, So I just today flasked some dendrobium anosmum and den. udom sri seeds. I flasked Cattleya walkeriana var. tipo 'cambara cell samples I took from an older severed back bulb as well. The cells and seeds were placed on agar in my flasking case with the air purifier on so I hope everything was sterile.
I DO NOT have an autoclave/pressure cooker as I just moved and would not know how to use it anyway. For me, micorwaving is the easiest way to do the agar and just do my best to keep contaminants out.
I put everything in the case and sprayed it all with disinfectant including the needles and syringes with cells and the containers full of seeds. But my question now is, what do i do???
I flasked and now do I just cross my fingers and hope that everything is sterile and the seeds are still viable? I am hoping to see some growth soon, but I know it can take anywhere from 2 weeks to 6 weeks for the seeds to START germination and cells might be a bit less. I guess now I have to play the waiting game.
I have the jars inside the flasking case and will keep them there and just use flourescent lights for 8-10 hours a day until i see green in the jars- I hope the good green and not the nasty green. I am keeping my fingers crossed as this will be my last attempt at in-vitro orchid culture- if I do not have luck with the seeds germinating and cells growing, then i give the hell up.
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