Flasking 101 - A how-to guide

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The handbook PROPAGATION OF CANADA'S ORCHIDS Methods and Issues by Marilyn H.S. Light has these recipies:

Oats Agar (for isolation of fungal symbionts and for symbiotic germination)

2.5 g oats (commercial rolled oats)
12 g agar powder
1000 ml deionized water

Water Agar (for symbiotic germination of Spiranthes sp., etc.)

8 g agar powder
1000 ml deionized water

Preperation is as for any other medium. Adjust ph as needed.

I'm not sure how well these recipies work as I've never actually attempted the symbiotic method. From what I've read, isolating the fungus can be tricky as other contaminants tend to overwhelm the culture before the desired symbioint. Once you're able to get a culture of the fungus you want going, you can take a piece of it and inoculate other flasks with the fungus for sowing. Again, I can't speak for the effectiveness of this method as I've never tried it.