Okay I wanted to start this page just as a little blog on what is going on with my stem propagation!! Because yes it is my first time and I hope it is helpful for others. First off in this page I will explain to you the steps I took and the test, well experiments that took place while in the process of stem propagation.
Well to start off I got some media which is suitable for stem propagation of Phalaenopsis's. In the process of making the agar media first off I only wanted 500 mL so I measured out half the required amount of media which was 12 .something grams. Then I mixed it in with sterilized water and stirred it around for a little bit after which I put it in the microwave for eight minutes. When I took it up the microwave I stirred it some more and then administered it to the little jars which are seen in the picture. I tried to put 25 mL but in the end it became more in some of the jars because I still had left overs.
Initially I made 22 jars but my pressure cooker is not big enough to fit them all in, so what I did was do a little experiment and I put 10 in the microwave and the other 12 in the pressure cooker. Well I placed the 10 in the microwave in there for 9 minutes with the lids but after they were done I quickly fastened lids. In the bottom of the pressure cooker I filled it with 1 inch to 2 inches of water and place most the jars on a metal pan that I had sitting a little bit above the water then I placed three on top of the rest of the jars. I put them in the pressure cooker for 30 minutes after which I fell a sleep and woke up I went to the kitchen and took them off the stove which had been turned off after 30 minutes I brought it into my laminar flow hood which was self built by a source.
Then I left them there for a day or two, maybe just a day. Then I went on orchid board and studied some, other places about techniques of sterilizing the stems.
So how mine goes is I take the cuttings of the plant no bigger than 2 inches. Then I put it in a jar with one drop of dish soap and regular tap water and shook it for about 5 minutes then I took them out from there and placed them a 10% bleach solution and left them in there for 15 minutes, which inside my laminar flow hood I used my sterilized scalpel and cut off the bracts and also I cut the stem down some on both sides above the node and below so the stem culture would be 3/4 of an inch to 1 inch. Then I put them in a 5% bleach solution for 10 minutes, shaking it every 2 to 3 minutes. This bleach solution should be discarded after that, and rinse them with sterilized water three times each time the solution should be discarded. And make sure to keep your utensils in alcohol, flaming them before uses and that was about it.
Actually in the end the microwave and the pressure cooker both came out sterilized. The only difference was the ones from the microwave had less agar in the end and the agar was harder. The ones sterilized in the pressure cooker was looser and looked like there was more in the jar, then when I started.
But I did this over a period of days, putting the actual stems in there!! Some were my newly purchased orchid's spikes!!
But one thing I did experiments on other than the microwave and the pressure cooker, was one of the jars from the microwave. I think the media boiled away almost all the way but there was still a little bit left. So I decided to put some microbials in there. I will add more later!! Hope you like it so far but I will edit it later this is the draft!! I would have took more pictures of the process but Iwasn't thinking about it aat that time!!! Sorry!
Stem Propagation with Ghosoff!
but it is a hepa filter and it has a squirl fan on the inside!!! Yeah i will try to take some more pictures in the morning because I am bout to do some other little tests but maybe when i am done I can do it probably but i will put it on tomorrow later ya'll!!
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