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11-13-2020, 03:48 PM
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Join Date: Mar 2020
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Adventures in Flasking
Last edited by Dorchid; 11-13-2020 at 04:03 PM..
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11-13-2020, 05:46 PM
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Fingers crossed for germination, and nicely explained!
I think it would be safer to rinse the seeds in sterile water, rather than keeping them in hydrogen peroxide. Even though it becomes water under light, long exposure time can damage seeds.
Second comment, 40 minutes of autoclaving seems overkill for the amount of medium in each flask. Chemicals contained in it are (mostly) heat sensitive.
If the lids are microwave-resistant, sterilising using a good ol' kitchen microwave saves a lot of time and trouble.
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11-13-2020, 05:57 PM
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Thanks Mr. F! Hopefully the components weren’t denatured with the extra time. I usually run 30-40 min on media sterilization which is indeed overkill even considering they aren’t for plant propagation. That said, I’d never use the microwave to sterilize media. I have another pod to harvest and will sterilize the media for 20 min. Yeah, I wasn’t too enthused with the H2O2 bath and would rather do a dilute bleach bath and sterile water rinse...I was lacking some things to carry out that procedure. The video I referenced purported good results so we’ll see what happens. Appreciate the input!
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11-14-2020, 07:45 PM
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08-13-2022, 01:10 PM
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The odcdm. project didn’t turn out. I didn’t get any germination from the seed as I think the ph of the medium was too low. I’m starting again with some noid phal seeds from a green capsule. The cap was washed in 50% Clorox solution for 25 min and rinsed multiple times with sterilized ro water. The contents were extremely fluffy and transferred to around 40ml of sterilized ro water and aspirated into a sterilized syringe. P723 was again used and I made 3 different batches. Two were ph 5.8, one with coconut water and the other without and the third was ph 5.6 with coconut water. PPM was used in all. 27 plates were poured and around 5 drops of seed solution applied to each. After distributing around the plate and allowing the seed to settle, the excess water was poured off and the plates wrapped in parafilm. They are currently in a tent with 16/8 moderate light (25W COB) with a temp range of around 65F-73F. All work was done in front of the hood.
Pollen parent
Cap parent
Cap at 6 months
Excess water poured off
Possible germination after 15 days
Homemade laminar flow station
Last edited by Dorchid; 08-13-2022 at 01:13 PM..
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08-13-2022, 06:01 PM
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Just found my loop and got a good look at the seeds. Those on the 5.6 ph formulation are swollen and green; Much more so than those on the 5.8.
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08-13-2022, 06:36 PM
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Love the detail and pictures! Great job with germination and good luck - I'm excited to see updates.
David
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08-13-2022, 11:06 PM
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Th8s is so far over my head, but it amazes me the a .2 difference in pH makes that much of a difference.
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08-13-2022, 11:35 PM
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Quote:
Originally Posted by Dusty Ol' Man
Th8s is so far over my head, but it amazes me the a .2 difference in pH makes that much of a difference.
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Remember, the pH scale is exponential.
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08-14-2022, 12:58 AM
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Quote:
Originally Posted by Roberta
Remember, the pH scale is exponential.
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logarithmic...meh, semantics, i think we get what you mean!
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