Seed flasking: lab having difficulty with disinfection

[Dorchid]

More? You contributed nothing. Are discussions and the sharing of information not to be organic and only facilitated in an institutional and utilitarian manner? I feel our conversation above is helpful in that it may contain information that op is not aware of, illustrate other approaches and possibly lead to the information they seek in a roundabout way. It doesn't answer op's question per se, but it is far from off-topic. Being passive aggressive, willfully obtuse and playing mod are more irritating and disruptive.

[Orchid Whisperer]

Originally Posted by Dorchid

More? You contributed nothing. Are discussions and the sharing of information not to be organic and only facilitated in an institutional and utilitarian manner? I feel our conversation above is helpful in that it may contain information that op is not aware of, illustrate other approaches and possibly lead to the information they seek in a roundabout way. It doesn't answer op's question per se, but it is far from off-topic. Being passive aggressive, willfully obtuse and playing mod are more irritating and disruptive.

This was posted about 2 minutes prior to my last post, therefore i didn't read it until after that post was made. There is no need to insult Ray for trying to focus back on the questions I asked; the insults are certainly off-topic, and not helpful.

[Oyarzabal]

@orchid whisper. If you are going the route of pre disinfecting the seeds. I read once a peer review paper about seed disinfection using chlorine gas and formaldehyde. The formaldehyde beat up Clorox in their experiment. I do not know if I read the paper in Spanish, Portuguese, or English. I will try to find it and post it.
I do not believe there will be bug able to escape a good disinfection. Aaron Hicks recommends to soak the seeds is a sugary solution for 24 hours before disinfection, I think it was 10% sugar. Gram negative will encapsulate themselves when things are not going well and can be hard to kill. They come out of their spores to feast in the sugar.
Soaking your seed in sugar water for 24h and them gassing them probably will solve your problem.

Found the paper.
http://citeseerx.ist.psu.edu/viewdoc...=rep1&type=pdf

I also read another paper about seed storage and there is direct relation between drying the seeds and mortality. So, the secret most be to strike a balance between dry enough not to encourage bacterial growth and not dry enough to kill the seeds. Low temperature also had a direct link to mortality in that paper. Steven Christoffersen told me he store his seed at around 68 Fahrenheit. And if that is good for him. I will go alone.

---------- Post added at 03:21 PM ---------- Previous post was at 03:05 PM ----------

Oops that was the same paper they use in the other discussion.
If you are using Clorox, this should be more effective method. He uses hydrochloric acid to release the gas in the Clorox solution. Language is a some kind of Slavic language, but it has English subtitles.