When to replate

[Afid]

They are doing pretty well! I will try to get some photos soon. There are now some roots on the larger seedlings, I still have a whole bunch in the original flasks that need to be moved out though, those are all much smaller. The only issue I have had is a few flasks got contaminated and there are some seedlings with yellowing leaves (not sure why this is).

[Dorchid]

Awesome! Are your jars vented? Pics!

[Afid]

Originally Posted by Dorchid

Awesome! Are your jars vented? Pics!

They are not vented unfortunately, I couldn't get my hands on any microfilters that would work. I will upload pics later today.

[Dorchid]

Right on. In the future, if you can’t get actual filters you can drill a 1/4” hole and pull tightly packed polyfill through. It makes a great filter. Other option would be synthetic filter disk glued with rtv which lasts many sterilization cycles. Both options should allow for ample gas exchange while preventing contams. At least they do in fungi culture.

[Afid]

here is a (unfortunately low quality) pic of one of the flasks, its hard to get good photos because of the thick glass.

[Dorchid]

Nice; those look great!

[Afid]

Originally Posted by Dorchid

Right on. In the future, if you can’t get actual filters you can drill a 1/4” hole and pull tightly packed polyfill through. It makes a great filter. Other option would be synthetic filter disk glued with rtv which lasts many sterilization cycles. Both options should allow for ample gas exchange while preventing contams. At least they do in fungi culture.

How do you sterilize the polyfill? I'm guessing it can't just go in the pressure cooker with the jars and lids.

[Dorchid]

Yep...it just needs changed out more often.

[Afid]

Originally Posted by Dorchid

Yep...it just needs changed out more often.

Cool, and just checking before I go and buy some for next time, this is the polyester fluff that people use to stuff pillows and things, right? Robot Check

[Dorchid]

That’s the stuff!

Also, what media are you using?