Tobacco Mosaic Virus is very similar to Cymbidium Mosaic Virus and Odontoglossum Ringspot Virus. I was looking for how to treat old LECA for safe reuse to prevent unrecognized viruses from spreading. I found the following. My conclusion is that pressure-cooking LECA at the high setting for 30 minutes (equivalent to 250 degrees F / 121C) should be more than enough.
http://www.jbc.org/content/140/2/509.full.pdf
THE DENATURATION OF TOBACCO MOSAIC VIRUS AT HIGH PRESSURES BY MAX A. LAUFFER AND R. B. DOW (From the Department of Animal and Plant Pathology of The Rockefeller Institute for Medical Research, Princeton, and the School of Chemistry and Physics, the Pennsylvania State College, State College) (Received for publication, May 19, 1941) Tobacco mosaic virus nucleoprotein (14), in common with many proteins, can be denatured by various means. A neutral [in pH] solution of the virus heated to about 70 [degrees C, 158 degrees F] will lose its infectivity rapidly and gradually become insoluble in dilute salt solutions.... If the virus solvent is made alkaline to a pH value of 10.5 or 11, this reaction will proceed at an appreciable rate even at room temperature.
Heat inactivation of viruses
Threaded Mode
