Tissue Culture General & Orchids

[kg5]

It is very obviously that tissue culture has made huge leaps in knowledge in recent years.

Keep getting caught out with old , out of date info.

The example I will use 1st is about orchids but I would like to talk about other plants as well so am using the off topic area to post this. The info will also be for all plants across the plant kingdom.

Have got the picture that recent developments have made diocotaleadons more user friendly to tissue culture endeavors other than locked into only using the meristem. Is this true?

Most of the info I have found is all about using the meristem but other info is telling me that fresh new leaf growth can be used because of the new medium available.

Can the new leaf/bulb growth that is about a 250mm or 10" long on a dendrobium speciosum be used for tissue culture?