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  #11  
Old 11-29-2020, 02:20 PM
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isurus79 isurus79 is offline
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I was looking into the literature surrounding the "runt" hypothesis that Roy has put forward. For reference he also is generally referencing looking through mericloned seedlings to find the polyploids arising from this cloning technique (in the context of his personal anecdote). This allows you to find one "type" of tetraploid as there is multiple mechanisms that result in a duplication of chromosomes. There is documentation that colchicine induced tetraploids in some plant types results in dwarfism and these plants have some disregulation with plant hormones because the doubling of the chromosome increases the amount of a regulatory mRNA which disrupts the signaling pathway. Additionally, runts may also be >4n which also results in other types of regulation of growth, so that precious tetraploid you think you have could just be a crazy chromosome mutant that will be cruddy its whole life.

Which is to say, all polyploids are not created equal and you are better off culling any weak plants, plants that lack characteristics of vigor, ect. than hoping for a rare tetraploid, which may or may not have anything good about it. This is why breeders have moved to oryzalin and colchicine treatment of protocorms because you can generate a lot of tetraploids with select plants and then select the best ones. This results in significantly more "useful" plants compared to the old needle and the haystack approach.
Very useful information! Good stuff, thanks!
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  #12  
Old 11-29-2020, 04:11 PM
thefish1337 thefish1337 is offline
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Method for rapid screening for tetraploids in Cattleya plantlet/seedlings. You can be reasonably assured that densities of stomata lower than 6/0.12mm^2 will be around 90% tetraploid.
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  #13  
Old 11-29-2020, 04:29 PM
Orchidtinkerer Orchidtinkerer is offline
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I have no knowledge of what 4N means even though I have some 4N seedlings - from what I have just read here it causes slow growth, dwarf plants, mutated flowers and has been forced onto some plants via some artifcial chemical.
I am well aware that is not going to be the true story, I though 4N made a plant stronger - I have a bit of understanding of plant biology from school but we never covered 4N!
Can I ask if someone knows does it actually occur naturally or is it something man-made?

Last edited by Orchidtinkerer; 11-29-2020 at 04:33 PM..
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Old 11-29-2020, 07:02 PM
Jeff214 Jeff214 is offline
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Oh im jealous of those seedlings! I emailed and called H&R a few times but I never got a response from them. Hope they do well for you!
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Old 11-29-2020, 08:08 PM
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isurus79 isurus79 is offline
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Originally Posted by Orchidtinkerer View Post
I have no knowledge of what 4N means even though I have some 4N seedlings - from what I have just read here it causes slow growth, dwarf plants, mutated flowers and has been forced onto some plants via some artifcial chemical.
I am well aware that is not going to be the true story, I though 4N made a plant stronger - I have a bit of understanding of plant biology from school but we never covered 4N!
Can I ask if someone knows does it actually occur naturally or is it something man-made?
Yes, 4N is a naturally occurrence. Chemicals are used now to increase the number of polyploid plants since they are so valuable due to the dramatically increased flower size.

---------- Post added at 06:08 PM ---------- Previous post was at 06:07 PM ----------

Quote:
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Oh im jealous of those seedlings! I emailed and called H&R a few times but I never got a response from them. Hope they do well for you!
Harry can take a little while to respond. It’s well worth the wait. He actually used to post here on OB as catwalker808.
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