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  #31  
Old 02-04-2013, 01:38 PM
DavidCampen DavidCampen is offline
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Quote:
Originally Posted by camille1585 View Post
And just a comment,while Agdia does sells the primers for it, it certainly is not for home use!
Speak for yourself, if I had another room I would consider setting up for doing this.

Quote:
The immunostrips don't exist yet for this virus, but a quick search of literature shows that the antiserum for it has been developped. So Elisa is possible.
Reference please.

---------- Post added at 09:31 AM ---------- Previous post was at 09:26 AM ----------

Quote:
Originally Posted by Leafmite View Post
I agree. I did quite a bit of asking and reading and what I learned is that it is recommended that a person is trained by someone experienced before that person begins testing plants or people for virus. If one doesn't perform the test perfectly, the results might not be perfect. Another test is always recommended to confirm results.
If a plant is vigorous, growing well, and exhibits no signs, how do you know, without an advanced test, whether something else is causing the false positive?
Basically, all of the above is wrong.

Quote:
Why toss an expensive orchid whithout certain confirmation it is virused? Why spend all that money for testing when you can just toss all the orchids and raise parakeets?
That is a bizarre statement.

Quote:
Seriously, if you observe your plants, I think it becomes clear pretty quickly if something is wrong with the plant. Buying from reputable vendors is very helpful, too.
An infected plant may be a genus, species or cultivar that it not seriously affected yet it is still capable of infecting another plant that will be seriously affected.

---------- Post added at 09:32 AM ---------- Previous post was at 09:31 AM ----------

Quote:
Originally Posted by ronaldhanko View Post
Please don't be so aggressive.
Why, have I hurt someone's feelings?

---------- Post added at 09:38 AM ---------- Previous post was at 09:32 AM ----------

Quote:
Originally Posted by camille1585 View Post
I never ever said that OSRV and CymMV viruses are benign, please don't make me say things I never wrote. I was writing about CABBAGE as an example and was simply illustrating that whether we like it or not, virus is everywhere. So chill a bit.
That is certainly what you were trying to imply when you said:

Quote:
The fact is that virus is everywhere in nature. I can across an article at work about the prevalence of virus in wild cabbage populations on the southern coast of England. 30 to 98% of the tested plants were positive of virus, with many of them positive for ALL 6 of the viruses tested for! Yet the plants are happily growing.
emphasis added is mine
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  #32  
Old 02-04-2013, 03:11 PM
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camille1585 camille1585 is offline
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I'm sorry, you are being agressive, tearing apart every single statement posted and picking at little details and inventing meanings to sentences.

The goal of this thread wasn't to take about viruses per say, but to discuss peoples opinions on virus in their collection. Which I thought was very interesting, since we've discussed the facts/science of virus more than once on OB. I understand and respect your zero tolerance attitide towards virus, you are entitled to feel that way. But I think you've more than gotten your point across now. Now please respect other people's opinions as well. And now, let's please get back to the origin topic of this thread.
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Last edited by camille1585; 02-04-2013 at 03:13 PM..
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  #33  
Old 02-04-2013, 03:48 PM
DavidCampen DavidCampen is offline
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Originally Posted by camille1585 View Post
I'm sorry, you are being agressive, tearing apart every single statement posted and picking at little details and inventing meanings to sentences.
Correcting erroneous statements is being aggressive? And your statement very well did have the meaning that I objected to.

But yes, I will stay out of this thread.

Last edited by DavidCampen; 02-04-2013 at 03:52 PM..
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  #34  
Old 02-04-2013, 04:06 PM
ronaldhanko ronaldhanko is offline
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No, David, you haven't hurt my feelings. I was only trying to point out (as Camille has also now done) that this was a friendly discussion of how we deal with the possibility that there are virused plants in our collections. In my case, for better or worse, ignorance is bliss. You, obviously, feel very strongly about this, and that's okay with me. I just didn't want you to get upset and anger people.

Last edited by ronaldhanko; 02-04-2013 at 04:54 PM..
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  #35  
Old 02-04-2013, 04:42 PM
Rowangreen Rowangreen is offline
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I don't see how Camille was trying to 'imply' certain viruses were harmless... never mind.

Here's a hypothetical example of typical hobby grower X. X has amassed a collection of 50 - 100 plants, without virus testing. Just for the sake of argument, let's assume every one of them is harbouring a virus. But they all appear healthy and are growing well. Now, hypothetically tomorow X is going to buy a new plant, which is actually free of the virus, and, what's more, will be badly affected by it and will die if not chucked first.

So, should X test every plant in their collection, ending up chucking all of them to save the one?

Or should they accept the occasional plant that dies?

It's one thing if you have a collection that you believe to be free of virus, or if you are intending to breed to sell. But for a hobby grower with a collection that seems healthy perhaps testing just isn't worth it.

PS: I'm about to try crossing two of my phals. I'm not intending to virus check them. If I succeed in getting enough seedlings to pass on I will either check them or let people know they've not been tested.
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  #36  
Old 02-04-2013, 05:17 PM
quiltergal quiltergal is offline
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[QUOTE=DavidCampen;549997]What basis do you have for making this statement? As far as I know that statement is not correct.[COLOR="Silver"]

Well, you yourself said in a different thread that there were several lots of strips from Agdia that were giving false positives. I see no reason to believe that it couldn't happen again. That is why I am sending my samples to a real lab. If your statement was incorrect then I will retract my statement.

There are nice ways of sharing an opinion.....and there is your way.
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  #37  
Old 02-04-2013, 06:24 PM
DavidCampen DavidCampen is offline
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[QUOTE=quiltergal;550080]
Quote:
Originally Posted by DavidCampen View Post
What basis do you have for making this statement? As far as I know that statement is not correct.[COLOR="Silver"]

Well, you yourself said in a different thread that there were several lots of strips from Agdia that were giving false positives. I see no reason to believe that it couldn't happen again. That is why I am sending my samples to a real lab. If your statement was incorrect then I will retract my statement.

There are nice ways of sharing an opinion.....and there is your way.
A _single_ batch of Agdia Immunostrips was reported to have been giving false positives on certain cymbidiums only and Agdia recalled those strips. My guess is that the false positives were the result of extreme incompetence on the part of some persons doing the test - like mistaking the control line for a virus signal. So yes, there probably are some people who can't properly perform a test as simple as using an Agdia Immunostrip.

Are you going to send samples to Critter Creek? They use immunoassy reagents from Agdia, essentially the same as are used for the Immunostrips.

Last edited by DavidCampen; 02-04-2013 at 06:27 PM..
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  #38  
Old 02-04-2013, 06:40 PM
Ordphien Ordphien is offline
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As far as the option part goes, I've personally never tested for a virus but I'm thinking about it.
My friend who had built up a collection of 100+ NOIDS decided to send in samples and have all her plants tested.
Her plants are all performing amazingly, so virus or not she had no intention of getting rid of them. But she did wanna know.
In the end only two came up positive for a virus.. these two were her best performing phals...
She kept them... still loves them...

On another note.. all her plants were from Trader Joe's... I found it really impressive that only two came in virused from such a large wholesaler.


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  #39  
Old 02-04-2013, 07:14 PM
escualida escualida is offline
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Quote:
Originally Posted by Leafmite View Post
Buying from reputable vendors is very helpful, too.
Unfortunately it's not easy to tell anymore which sellers are good about this and which aren't. I bought 7 plants from Pine Ridge thinking they were a "reputable" seller and 6 of them came back positive for both ORSV and CymMV. When I let them know they replied back with indifference and basically told me I was screwed. The plants I bought were expensive and some of them were even ones that they used for breeding, I never expected them to be infected with a virus, let alone with both. From this experience it is clear to me that they don't care about viruses one bit, and now I know. But I couldn't have known that had I not tested.

Quote:
Originally Posted by Rowangreen View Post
PS: I'm about to try crossing two of my phals. I'm not intending to virus check them. If I succeed in getting enough seedlings to pass on I will either check them or let people know they've not been tested.
Unfortunately if the one you choose as the pollen parent is virused and the one you choose as the pod parent is not, you'll likely spread that virus to the uninfected plant. And how would you know unless you get them tested?

If that's still a risk you're willing to take you could try using dry seed instead of green pod when it comes time to flask them, this will make it much more likely that the seedlings will be virus-free. This is what I'm going to have to do with the plants that I mentioned above, I'm actually going to self them as well so that I have backups of the sick ones which will inevitably die.
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  #40  
Old 02-04-2013, 08:19 PM
WhiteRabbit WhiteRabbit is offline
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ENOUGH! AGAIN! Apparently asking people nicely to please keep things friendly is not sufficient! Seriously - I don't want to be the person giving infractions - but I don't know what else to do. I WILL give infractions, and do not feel the need to give anyone a private warning nor explanation.

It's fine to explain why one does, or does not test every plant they have - but we all should be able to do so without snark towards others.

I was locking this, but am un-locking - BEHAVE! Mods will be keeping an eye on this.

Last edited by WhiteRabbit; 02-04-2013 at 08:57 PM..
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