Meristem cloning

[tulasi132]

Wow. All this is awesome. Thank you.

[evaluna]

There is a good article on Meristem cloning in a book by Steven A. Frownie called "Moth Orchids, a complete guide to Phalaenopsis". Good Luck!

[Royal]

My understanding is that constant agitation helps proliferation. As someone stated, when inducing callus tissue these cells are undifferentiated. We don't have to worry about gravity at this point - just making more, and more, and more protocorm-like bodies.

An orchid "factory" wants millions of plants. For a "garage project" I'd bet you don't even need a rotator. You'd probably get plenty of proliferation in the right liquid media.

[orchidsamore]

Here is a video of a cloning laboratory that I have used.

YouTube - AgriStarts Tour

What was most interesting was the need to re-plate plants every four weeks.

I was surprised to learn when talking to the owners that when plants are grown from seeds the lab can keep separating the plants in the flasks and keep the original strain propagating forever. Production is not limited to the number of seeds in the pod.

[MRay]

Most tissues in a liquid system need some form of aeration be it shaking, rocking, bubbling, temporary immersion, or rafting. Otherwise, waterlogging can easily occur. There are plenty of exceptions. However, the orchids that I have seen grown in a liquid system have some method of aeration.